Figure 4

Phosphorylation on GluA2-S880, but not on GluA2-Y869/Y873/Y876, is impaired in Ptprr ^−/− cerebellar slices upon LTD-induction. (A) Immunoblot detecting phospho-S880-GluA2 (upper panel) and total GluA2 (lower panel) in cerebellar slices of wild-type and Ptprr ^−/− mice under basal conditions, stimulated with K-Glu to induce LTD, or after subsequent wash out of K-Glu for 10 or 20 min. (B) Quantitative representation of the relative phospho-GluA2 levels, calculated as the ratio of phospho-GluA2 over total GluA2 levels (arbitrary units) from the different cerebellar slice lysates as described under (A). Results are presented as mean values and error bars indicate SEM from five independent experiments (ANOVA repeated measures, *p < 0.05). (C) Phospho-Y869/Y873/Y876-GluA2 (upper panel) and GAPDH (lower panel) immunoblot detection in wild-type and Ptprr ^−/− cerebellar slices under basal conditions, stimulated with K-Glu to induce LTD, or after subsequent washes of 10 or 20 min. (D) Quantitative representation of the relative p3Y-GluA2 levels, normalized over GAPDH levels (arbitrary units) from the different cerebellar slice lysates as described under (C). Results are presented as mean values and error bars indicate SEM from four independent experiments (ANOVA repeated measures, *p < 0.02).