Impairment of cerebellar LTD in
Purkinje cells does not result from Src-family kinase hyperactivity. (A) Averaged time course of PF-EPSC amplitudes triggered by PF&ΔV and normalized to their mean prestimulation level, recorded from PCs in wild-type (n = 3) or Ptprr
−/− (n = 3) cerebellar slices while including a Src family kinase inhibitor (PP1 analog) in the patch pipet. (B) Wild-type and Ptprr
−/− cerebellar slices under basal conditions,stimulated with K-Glu to induce LTD or after subsequent washes of 10 or 20 min were used for immunoblot analysis of Src kinase activity, using phospho-Y416-Src (upper panel set) and total Src (lower panel set) antibodies. GAPDH immunostaining (lower panel in each set) was used for normalization purposes. Representative images out of four independent experiments are shown.