Increased numbers of differentiating NSPCs in SVZ of G93A-SOD1 brain. (A) Confocal microscopy images of dorsal and ventral regions of SVZ in G93A-SOD1 and Wt-SOD1 brain at postnatal week 18, stained for nestin (green), GFAP (red), and vimentin (blue). Scale bar = 50 μm. (B) Quantification of nestin-, GFAP-, and vimentin-positive cells in SVZ of G93A-SOD1 and Wt-SOD1 mice. Data are means ± SD of 3 mice per group. **p < 0.001; limma moderated t-test. (C) Hematoxylin and eosin staining of SVZ sections adjacent to those analyzed by confocal microscopy. Scale bar = 50 μm. D: dorsal. V: ventral. (D) Confocal microscopy images showing Dlx2-stained cells in ventral SVZ in G93A-SOD1 and Wt-SOD1 brain. Scale bar = 50 μm. (E) Quantification of Dlx2-positive cells in G93A-SOD1 and Wt-SOD1 SVZ. Data are means ± SD of 3 mice per group. **p < 0.001; limma moderated t-test.