Table 2 Reagents, source companies and working dilutions
Source | |||
|---|---|---|---|
Animals | G93A-SOD1 (B6SJL-Tg(SOD1*G93A)1Gur) | Charles River (Wilmington, MA) | |
Wild-type (Wt)-SOD1 (B6SJLTg(SOD1)2Gur/J) | Charles River (Wilmington, MA) | ||
B6.SJL | Charles River ((Wilmington, MA) | ||
Working dilution | |||
Primary | -Rabbit anti-mouse glial fibrillary acidic protein | ||
Antibodies | (GFAP) IgG antibody | Dako Cytomation (Glostrup, Denmark) | 1:300 |
-Chicken anti-mouse vimentin IgG antibody | Novus Biological | 1:500 | |
-Mouse anti-mouse nestin IgG antibody | Millipore (Billerica, MA) | 1:200 | |
-Goat anti-mouse Dlx2 IgG antibody | Santa Cruz (Heidelberg, Germany) | 1:50 | |
Secondary | -Cy3-conjugated goat anti-rabbit IgG | Jackson ImmunoResearch (Newmarket, UK) | 1:600 |
antibodies | -AMCA-conjugated anti-chicken IgG | Jackson ImmunoResearch (Newmarket, UK) | 1:100 |
-Cy2-conjugated goat anti-mouse IgG | Jackson ImmunoResearch (Newmarket, UK) | 1:200 | |
-Cy2-conjugated donkey anti-goat IgG | Jackson ImmunoResearch (Newmarket, UK) | 1:200 | |
-Isotype-specific non-immune IgG (control) | Dako Cytomation (Glostrup, Denmark) | 1:200 | |
-Normal goat serum (control) | Vector Laboratories (Peterborough, UK) | ||
Reagents for | -Optical Cutting Temperature Compound | Bio-Optica (Milan, Italy) | |
sample conservation | |||
Microscopes | -Aperio scanner | Nikon (GMBH, Germany) | |
-Aperio Image Scope v12.0.0.5039 | Nikon (GMBH, Germany) | ||
-Eclipse TE-2000-E | Nikon (Tokyo, Japan) | ||
Reagents | -Trizol | Life Technologies (Foster City, MA) | |
qReal-Time PCR | -2100Nano Bioanalyzer | Agilent Technologies (Waldbronn, Germany) | |
-TaqMan MicroRNA reverse Transcription Kit | Life Technologies (Foster City, MA) | ||
-miRBase ID mmu-miR-9-5p | Life Technologies (Foster City, MA) | ||
-miRBase ID mmu-miR-124-3p | Life Technologies (Foster City, MA) | ||
-miRBase ID mmu-miR-134-5p | Life Technologies (Foster City, MA) | ||
-miRBase ID mmu-miR-125b-5p | Life Technologies (Foster City, MA) | ||
-miRBase ID mmu-miR-219a-5p | Life Technologies (Foster City, MA) | ||
-miRBase ID mmu-miR-19a-3p | Life Technologies (Foster City, MA) | ||
-miRBase ID mmu-miR-19b-3p | Life Technologies (Foster City, MA) | ||
-miRBase ID xtr-miR-24a-3p | Life Technologies (Foster City, MA) | ||
Reagents for | |||
miRNA | -SuperScript Vilo cDNA Synthesis kit | Life Technologies (Foster City, MA) | |
target validation | -Universal PCR master mix | Life Technologies (Foster City, MA) | |
-Taqman Array Fast Plate | Life Technologies (Foster City, MA) | ||
-Mm-Ccnd2-Assay ID00438070_m1 | Life Technologies (Foster City, MA) | ||
-Mm-Dlx2-Assay ID00438427_m1 | Life Technologies (Foster City, MA) | ||
-Mm-Foxj3-Assay ID00554610_m1 | Life Technologies (Foster City, MA) | ||
-Mm-Hes1-Assay ID01342805_m1 | Life Technologies (Foster City, MA) | ||
-Mm-Jag1-Assay ID00496902_m1 | Life Technologies (Foster City, MA) | ||
-Mm-Mknk2- Assay ID00458026_m1 | Life Technologies (Foster City, MA) | ||
-Mm-Nr2e1-Assay ID00438427_m1 | Life Technologies (Foster City, MA) | ||
-Mm-Pten-Assay ID00477208_m1 | Life Technologies (Foster City, MA) | ||
-Mm-Smurf1-Assay ID00547102_m1 | Life Technologies (Foster City, MA) | ||
-Mm-Socs1-Assay ID01219775_g1 | Life Technologies (Foster City, MA) | ||
-Mm-Sox6-Assay ID00488393_m1 | Life Technologies (Foster City, MA) | ||
-Mm-Sox9-Assay ID00448840_m1 | Life Technologies (Foster City, MA) | ||
-Mm-Stat3-Assay ID01219775_m1 | Life Technologies (Foster City, MA) | ||
-Mm-GFAP-Assay ID0125033_m1 | Life Technologies (Foster City, MA) | ||
-Mm-18 s-Assay ID03928990_g1 | Life Technologies (Foster City, MA) | ||