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Table 2 Reagents, source companies and working dilutions

From: Up-regulation of neural and cell cycle-related microRNAs in brain of amyotrophic lateral sclerosis mice at late disease stage

Source
Animals G93A-SOD1 (B6SJL-Tg(SOD1*G93A)1Gur) Charles River (Wilmington, MA)  
  Wild-type (Wt)-SOD1 (B6SJLTg(SOD1)2Gur/J) Charles River (Wilmington, MA)  
  B6.SJL Charles River ((Wilmington, MA)  
    Working dilution
Primary -Rabbit anti-mouse glial fibrillary acidic protein   
Antibodies (GFAP) IgG antibody Dako Cytomation (Glostrup, Denmark) 1:300
  -Chicken anti-mouse vimentin IgG antibody Novus Biological 1:500
  -Mouse anti-mouse nestin IgG antibody Millipore (Billerica, MA) 1:200
  -Goat anti-mouse Dlx2 IgG antibody Santa Cruz (Heidelberg, Germany) 1:50
Secondary -Cy3-conjugated goat anti-rabbit IgG Jackson ImmunoResearch (Newmarket, UK) 1:600
antibodies -AMCA-conjugated anti-chicken IgG Jackson ImmunoResearch (Newmarket, UK) 1:100
  -Cy2-conjugated goat anti-mouse IgG Jackson ImmunoResearch (Newmarket, UK) 1:200
  -Cy2-conjugated donkey anti-goat IgG Jackson ImmunoResearch (Newmarket, UK) 1:200
  -Isotype-specific non-immune IgG (control) Dako Cytomation (Glostrup, Denmark) 1:200
  -Normal goat serum (control) Vector Laboratories (Peterborough, UK)  
Reagents for -Optical Cutting Temperature Compound Bio-Optica (Milan, Italy)  
sample conservation    
Microscopes -Aperio scanner Nikon (GMBH, Germany)  
  -Aperio Image Scope v12.0.0.5039 Nikon (GMBH, Germany)  
  -Eclipse TE-2000-E Nikon (Tokyo, Japan)  
Reagents -Trizol Life Technologies (Foster City, MA)  
qReal-Time PCR -2100Nano Bioanalyzer Agilent Technologies (Waldbronn, Germany)  
  -TaqMan MicroRNA reverse Transcription Kit Life Technologies (Foster City, MA)  
  -miRBase ID mmu-miR-9-5p Life Technologies (Foster City, MA)  
  -miRBase ID mmu-miR-124-3p Life Technologies (Foster City, MA)  
  -miRBase ID mmu-miR-134-5p Life Technologies (Foster City, MA)  
  -miRBase ID mmu-miR-125b-5p Life Technologies (Foster City, MA)  
  -miRBase ID mmu-miR-219a-5p Life Technologies (Foster City, MA)  
  -miRBase ID mmu-miR-19a-3p Life Technologies (Foster City, MA)  
  -miRBase ID mmu-miR-19b-3p Life Technologies (Foster City, MA)  
  -miRBase ID xtr-miR-24a-3p Life Technologies (Foster City, MA)  
Reagents for    
miRNA -SuperScript Vilo cDNA Synthesis kit Life Technologies (Foster City, MA)  
target validation -Universal PCR master mix Life Technologies (Foster City, MA)  
  -Taqman Array Fast Plate Life Technologies (Foster City, MA)  
  -Mm-Ccnd2-Assay ID00438070_m1 Life Technologies (Foster City, MA)  
  -Mm-Dlx2-Assay ID00438427_m1 Life Technologies (Foster City, MA)  
  -Mm-Foxj3-Assay ID00554610_m1 Life Technologies (Foster City, MA)  
  -Mm-Hes1-Assay ID01342805_m1 Life Technologies (Foster City, MA)  
  -Mm-Jag1-Assay ID00496902_m1 Life Technologies (Foster City, MA)  
  -Mm-Mknk2- Assay ID00458026_m1 Life Technologies (Foster City, MA)  
  -Mm-Nr2e1-Assay ID00438427_m1 Life Technologies (Foster City, MA)  
  -Mm-Pten-Assay ID00477208_m1 Life Technologies (Foster City, MA)  
  -Mm-Smurf1-Assay ID00547102_m1 Life Technologies (Foster City, MA)  
  -Mm-Socs1-Assay ID01219775_g1 Life Technologies (Foster City, MA)  
  -Mm-Sox6-Assay ID00488393_m1 Life Technologies (Foster City, MA)  
  -Mm-Sox9-Assay ID00448840_m1 Life Technologies (Foster City, MA)  
  -Mm-Stat3-Assay ID01219775_m1 Life Technologies (Foster City, MA)  
  -Mm-GFAP-Assay ID0125033_m1 Life Technologies (Foster City, MA)  
  -Mm-18 s-Assay ID03928990_g1 Life Technologies (Foster City, MA)