|
Animals
|
G93A-SOD1 (B6SJL-Tg(SOD1*G93A)1Gur)
|
Charles River (Wilmington, MA)
| |
| |
Wild-type (Wt)-SOD1 (B6SJLTg(SOD1)2Gur/J)
|
Charles River (Wilmington, MA)
| |
| |
B6.SJL
|
Charles River ((Wilmington, MA)
| |
| | | |
Working dilution
|
|
Primary
|
-Rabbit anti-mouse glial fibrillary acidic protein
| | |
|
Antibodies
|
(GFAP) IgG antibody
|
Dako Cytomation (Glostrup, Denmark)
|
1:300
|
| |
-Chicken anti-mouse vimentin IgG antibody
|
Novus Biological
|
1:500
|
| |
-Mouse anti-mouse nestin IgG antibody
|
Millipore (Billerica, MA)
|
1:200
|
| |
-Goat anti-mouse Dlx2 IgG antibody
|
Santa Cruz (Heidelberg, Germany)
|
1:50
|
|
Secondary
|
-Cy3-conjugated goat anti-rabbit IgG
|
Jackson ImmunoResearch (Newmarket, UK)
|
1:600
|
|
antibodies
|
-AMCA-conjugated anti-chicken IgG
|
Jackson ImmunoResearch (Newmarket, UK)
|
1:100
|
| |
-Cy2-conjugated goat anti-mouse IgG
|
Jackson ImmunoResearch (Newmarket, UK)
|
1:200
|
| |
-Cy2-conjugated donkey anti-goat IgG
|
Jackson ImmunoResearch (Newmarket, UK)
|
1:200
|
| |
-Isotype-specific non-immune IgG (control)
|
Dako Cytomation (Glostrup, Denmark)
|
1:200
|
| |
-Normal goat serum (control)
|
Vector Laboratories (Peterborough, UK)
| |
|
Reagents for
|
-Optical Cutting Temperature Compound
|
Bio-Optica (Milan, Italy)
| |
|
sample conservation
| | | |
|
Microscopes
|
-Aperio scanner
|
Nikon (GMBH, Germany)
| |
| |
-Aperio Image Scope v12.0.0.5039
|
Nikon (GMBH, Germany)
| |
| |
-Eclipse TE-2000-E
|
Nikon (Tokyo, Japan)
| |
|
Reagents
|
-Trizol
|
Life Technologies (Foster City, MA)
| |
|
qReal-Time PCR
|
-2100Nano Bioanalyzer
|
Agilent Technologies (Waldbronn, Germany)
| |
| |
-TaqMan MicroRNA reverse Transcription Kit
|
Life Technologies (Foster City, MA)
| |
| |
-miRBase ID mmu-miR-9-5p
|
Life Technologies (Foster City, MA)
| |
| |
-miRBase ID mmu-miR-124-3p
|
Life Technologies (Foster City, MA)
| |
| |
-miRBase ID mmu-miR-134-5p
|
Life Technologies (Foster City, MA)
| |
| |
-miRBase ID mmu-miR-125b-5p
|
Life Technologies (Foster City, MA)
| |
| |
-miRBase ID mmu-miR-219a-5p
|
Life Technologies (Foster City, MA)
| |
| |
-miRBase ID mmu-miR-19a-3p
|
Life Technologies (Foster City, MA)
| |
| |
-miRBase ID mmu-miR-19b-3p
|
Life Technologies (Foster City, MA)
| |
| |
-miRBase ID xtr-miR-24a-3p
|
Life Technologies (Foster City, MA)
| |
|
Reagents for
| | | |
|
miRNA
|
-SuperScript Vilo cDNA Synthesis kit
|
Life Technologies (Foster City, MA)
| |
|
target validation
|
-Universal PCR master mix
|
Life Technologies (Foster City, MA)
| |
| |
-Taqman Array Fast Plate
|
Life Technologies (Foster City, MA)
| |
| |
-Mm-Ccnd2-Assay ID00438070_m1
|
Life Technologies (Foster City, MA)
| |
| |
-Mm-Dlx2-Assay ID00438427_m1
|
Life Technologies (Foster City, MA)
| |
| |
-Mm-Foxj3-Assay ID00554610_m1
|
Life Technologies (Foster City, MA)
| |
| |
-Mm-Hes1-Assay ID01342805_m1
|
Life Technologies (Foster City, MA)
| |
| |
-Mm-Jag1-Assay ID00496902_m1
|
Life Technologies (Foster City, MA)
| |
| |
-Mm-Mknk2- Assay ID00458026_m1
|
Life Technologies (Foster City, MA)
| |
| |
-Mm-Nr2e1-Assay ID00438427_m1
|
Life Technologies (Foster City, MA)
| |
| |
-Mm-Pten-Assay ID00477208_m1
|
Life Technologies (Foster City, MA)
| |
| |
-Mm-Smurf1-Assay ID00547102_m1
|
Life Technologies (Foster City, MA)
| |
| |
-Mm-Socs1-Assay ID01219775_g1
|
Life Technologies (Foster City, MA)
| |
| |
-Mm-Sox6-Assay ID00488393_m1
|
Life Technologies (Foster City, MA)
| |
| |
-Mm-Sox9-Assay ID00448840_m1
|
Life Technologies (Foster City, MA)
| |
| |
-Mm-Stat3-Assay ID01219775_m1
|
Life Technologies (Foster City, MA)
| |
| |
-Mm-GFAP-Assay ID0125033_m1
|
Life Technologies (Foster City, MA)
| |
| |
-Mm-18 s-Assay ID03928990_g1
|
Life Technologies (Foster City, MA)
| |
