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Figure 1 | Molecular Brain

Figure 1

From: Ontogeny of biochemical, morphological and functional parameters of synaptogenesis in primary cultures of rat hippocampal and cortical neurons

Figure 1

Ontogeny of synaptophysin expression level in hippocampal and cortical cell cultures. The level of synaptophysin expression increases with time in primary cultures of rat cortical and hippocampal neurons. Dissociated cell cultures were derived from the hippocampi and neocortices of rat pups on postnatal day 1 (P1). Cultures were either fixed and immunostained for synaptic proteins (A, C, D), or cell lysates were collected for synaptophysin ELISA (B) at days in vitro (DIV) 7, 14, 21 and 28. (A) Representative fluorescence photomicrographs of synaptophysinsyn immunoreactivity at differing DIV in cortical or hippocampal neurons. Bar = 50 μm. (B) Relative ELISA levels confirm the increase in synaptophysin levels with increasing time in culture in both hippocampal and cortical neurons. AU = Arbitrary Units. Experiments were repeated >3 times with independent biological replicates. Data presented as the mean ± SD. Two-way ANOVA demonstrated a significant main effect of time and no main effect of cell type, therefore data from both cell types were collapsed within each time point for post-hoc analysis. *Significantly different from measurements at 7 DIV (Sidak’s post-hoc test, p < 0.05). (C, D) Representative fluorescence photomicrographs of a cortical (C) and hippocampal (D) neuron triple-labeled for synaptophysin, vGLUT1 and vGAT immunoreactivity at 21 DIV. Bar = 30 μm.

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