Figure 2From: The production of viral vectors designed to express large and difficult to express transgenes within neurons Vector maps of AAV vectors optimized for large transgenes. Viral genome maps for pAAV vector backbones containing either the Flag-GluN2A/B or GFP transgenes. Each of these vectors contain a ~75 bps 3′UTR that contains an SV40 based poly-adenylation signal. These transgenes are controlled by one of the following promoters as indicated: 1.3αCaMKII, 0.4αCaMKII, 1.1Synapsin, 0.5Synapsin, CMV, TRE3G, EFS, or RSV. The promoters are depicted as their approximate relative sizes to one another. GFP = green fluorescence protein, ITR = inverted terminal repeat, MCS = multiple cloning site.Back to article page