Figure 1

Overview of double immunostaining design. (A) Diagram of the initial experiments comparing early proliferation of astrocytes (BrdU/GFAP-positive) at 1Â week and mature neuron formation at 4Â weeks (BrdU/NeuN-positive) after middle cerebral artery occlusion (MCAO). (B) Coronal sections delineating the main areas of interest: frontal and temporal boundary zone.