Immunohistochemical (IHC) staining for anti-nestin in the peri-infarct area at 3, 7, and 14 days after ischemia (magnification, A 40×; B 400×). Compared with the corresponding regions of the control group, there was an increase in nestin-positive cells in the aLA group at 3, 7, and 14 days after the MCAO. Nestin-positive cells in the aLA group exhibited a variety of morphological features such as stellar shape, large cell body, and multiple processes (arrow). In contrast, stained cells in the control group exhibited small cell bodies, vacuolization, degradation, and loss of processes (arrowhead). n = 3 rats/day/group.