Immunofluorescence for BrdU (red) and GFAP (green). aLA increased the absolute number of newly developing astrocytes (BrdU/GFAP-positive) in the frontal and temporal boundary zone at 7 days after ischemia (A and D). Bar graph showing the absolute numbers of newly developing glia (BrdU/GFAP-positive) in the brain (B, C, E, and F; *P < 0.05; †P < 0.01 when compared with the control group; Student’s unpaired t test). Data represents mean ± SEM (n = 4 rats/day/group). Scale bars = 100 μm.