Immunohistochemistry for DCX (red) and BrdU (green). aLA treatment increased early neurogenesis (BrdU/DCX-positive) in the frontal (A) and temporal (C) boundary zone at 2 weeks after stroke. Bar graph showing the absolute numbers of co-labeled cells (BrdU/DCX-positive) in the brain (B and D; *P < 0.05; †P < 0.01 when compared with the control group; Student’s unpaired t test). Data represents mean ± SEM (n = 4 rats/day/group). Scale bars = 50 μm.