Carvacrol (CAR) pre-treatment reduced infarct volume of neonatal hypoxic-ischemic brain injury in a dose-dependent manner. A, TTC staining was performed as described in methods section. The representative images of TTC-stained coronal brain slices are shown (Left panel). Infarct volume is smaller in carvacrol pre-treatment group than vehicle group (*, p < 0.05 compared with vehicle group. #, p < 0.05 compared with carvacrol 30 mg/kg group, One-way ANOVA followed by Newman-Keuls test). B, representative images of whole brain and Nissl staining for brain slices (sham, n = 7; vehicle group, n = 12; carvacrol pretreatment group, n = 15).