TRPM7 protein expression in the cortex of HI mice and TUNEL staining of mouse brain slices. A, western blotting results of TRPM7 protein expression in the injured hemisphere (ipsilateral) and uninjured contralateral hemisphere 24 hours after HI. TRPM7 protein expression level is higher in injured hemisphere than the contralateral hemisphere (*, p < 0.05, n = 4). B, TUNEL staining was carried out 3 days after HI and images were viewed using fluorescence microscopy. Left panel shows the representative images of TUNEL staining. Red fluorescence in TUNEL-positive cells indicates apoptosis. Blue fluorescence is DAPI staining for nucleus. Scale bar represents 10 μm. Right panel shows the mouse brain coronal sections. C, Quantitative analysis of TUNEL-positive cells showed that pre-treatment with carvacrol (50 mg/kg) reduced the number of TUNEL positive cells (*, p < 0.05 compared with sham group. #, p < 0.05 compared with vehicle group, n = 3, One-way ANOVA followed by Newman-Keuls test).