Western blotting results. Experiments were carried out as described in the methods section. A, representative images of Bcl-2 (26 kDa), Bax (20 kDa), cleaved caspase-3 (19/17 kDa), p-Akt (Ser 473, 60 kDa) and t-Akt (60 kDa) protein expression. β-actin (42 kDa) was used as a loading control. Carvacrol (50 mg/kg) pretreatment increased the protein ratio of Bcl-2/Bax (B), decreased cleaved caspase-3 protein expression (C) and increased p-Akt/t-Akt protein ratio (D) 24 hours after HI (*, p < 0.05 versus sham group; #, p < 0.05 versus vehicle group, n = 3, One-way ANOVA followed by Newman-Keuls test). When primary mouse cortical neurons were exposed to OGD for 60 minutes and additional 18 hours in normal culture medium, Bcl-2/Bax ratio in the OGD group was lower than that of the control group, and significantly increased in the Carvacrol (300 μM) pre-treatment group (E and F, *, p < 0.05 versus the sham group; #, p < 0.05 versus the vehicle group, n = 3, One-way ANOVA followed by Newman-Keuls test).