Figure 5

Expression of HCN4 in PV-positive cells of the hippocampus at P21. (A-B, D-F, H-J, L-N, P): The images show HCN4 labeling in different fields of the hippocampus and the DG. (A, E, I): Note that HCN4 expression was most prominent in the sl-m and sp of the CA1 (A) and CA2 (E), and in the sl of the CA3 (I); but it was absent from the alveus. In addition, most PV-immunopositive cells located in the sp of the CA1, the so and sp of the CA2, sl, sp, and so of the CA3, and the hilus of the DG also co-expressed HCN4. A-B, D-F, H-J, L-N, P: white arrows indicate PV-positive/HCN4-positive cells. C, G, K, O: DAPI staining. Scale bars = 20 μm.