Figure 7
Expression of HCN1 in PV-positive cells of the hippocampus at P56. (A, E, I): The strongest labeling for HCN1 was observed in the sl-m and alveus of the CA. Note also that its expression was gradually stronger in the sp, and sr of the CA2 (E) and CA3 (I) compared to the CA1 (A). (A, B, D): PV-positive cells located in the sp of the CA1 did not show HCN1 immunolabeling. On the other hand, in the CA2, CA3, and hilus of the DG most PV-immunopositive cells were labeled with HCN1. A-B, D: yellow arrows indicate PV-positive/HCN1-negative cells. E-F, H-J, L-N, P: white arrows indicate PV-positive/HCN1-positive cells. C, G, K, O: DAPI staining. Scale bars = 20 μm.