Figure 8

Expression of HCN2 in PV-positive cells of the hippocampus at P56. (A-B, D, E-F, H-J, L-N, P): Most PV-immunopositive cells located in the CA1 (A-B, D), CA2 (E, F, H), CA3 (I, J, L), and DG (M, N, P) were labeled with HCN2. (A, E, I): HCN2 expression was most prominent in the sl-m of the CA1 (A), as well as in the sp of the CA2 (E) and CA3 (I). Interestingly, at this stage the alveus showed HCN2 labeling, contrasting with its expression pattern at P21. A-B, D, E-F, H-J, L-N, P: white arrows indicate PV-positive/HCN2-positive cells. C, G, K, O: DAPI staining. Scale bars = 20 μm.