Figure 5

Effects of bilateral elPB inactivation on responses to electrical foot-shock, thermal stimuli, and mechanical stimuli. A, Summary of the stimulation thresholds for responses to flinch (left), vocalization (middle), and jump (right) in the foot-shock sensitivity test with increasing stepwise US foot-shock intensities (mA). There were no significant group differences between the MUS group (n = 12), the drug control (n = 10), and the placement control (n = 9) groups. B, Summary of the paw withdrawal threshold to mechanical stimuli determined by the von Frey filament test. There were no significant differences in mechanical thresholds between the groups. The threshold is shown as the average of right and left paw thresholds. C, Summary of the latency to thermal stimuli examined by the hot plate test. No significant group differences were observed. D, Histological identification of injection sites. Injection sites were indicated in the same manner as those in Figure 2C for all animals used for the stimulus sensitivity analyses. Numbers on the left indicate antero-posterior levels relative to the bregma.