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Figure 1 | Molecular Brain

Figure 1

From: Ca2+/Calmodulin-dependent protein Kinase II interacts with group I Metabotropic Glutamate and facilitates Receptor Endocytosis and ERK1/2 signaling: role of β-Amyloid

Figure 1

Co-immunoprecipitation of GFP-CaMKIIα with FLAG-mGluR1a and FLAG-mGluR5a. Representative immunoblot showing GFP-CaMKIIα co-immunoprecipitation with either A) FL-mGluR1a or B) FL-mGluR5a from HEK 293 cells transiently transfected as labeled with 3 μg of pcDNA3.1 of either FL-mGluR1a or FL-mGluR5a along with either 0.5 μg of plasmid cDNA encoding either pEGFP or GFP-CaMKIIα and treated with 50 μM quisqualate for the times indicated in the Figure. Bar graphs show the quantitative densitometric analysis of GFP-CaMKIIα co-immunoprecipitated with either FL-mGluR1a or FL-mGluR5a. The data represents the mean ± SD of 6 independent experiments. C) Representative immunoblot showing GFP-CaMKIIα co-immunoprecipitation with FLAG-mGluR5a in HEK 293 cells treated with or without 5 μM KN-93 that were transiently transfected with 2 μg of FL-mGluR5 along with 0.5 μg of GFP-CaMKIIα. Bar graphs show the quantitative densitometric analysis of GFP-CaMKIIα co-immunoprecipitated with FL-mGluR5a in the absence and following pretreatment with 5 μM KN-93 for 1 h. The data represents the mean ± SD of 6 independent experiments. D) Shown is a representative immunoblot of endogenous CaMKII co-immunoprecipitated with endogenous mGluR5. 1 mg of adult CD-1 mouse hippocampal tissue lysate was incubated with protein G sepharose beads and either polyclonal rabbit anti-Rab11 or anti-mGluR5 antibody. Shown is a representative immunoblot from 4 independent experiments.

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Molecular Brain

ISSN: 1756-6606