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Figure 3 | Molecular Brain

Figure 3

From: Ca2+/Calmodulin-dependent protein Kinase II interacts with group I Metabotropic Glutamate and facilitates Receptor Endocytosis and ERK1/2 signaling: role of β-Amyloid

Figure 3

CaMKIIα overexpression increases agonist-stimulated mGluR1a and mGluR5 endocytosis. A) Upper blot shows a representative immunoblot for cell surface biotin-labeled mGluR1a in HEK 293 cells transfected with 3 μg of pcDNA3.1 encoding FL-mGluR1a along with 0.5 μg of plasmid cDNA encoding either GFP or GFP-CaMKIIα following 50 μM quisqualate treatment following 1 h pre-treatment in the presence and absence of KN-93. Lower blot shows the total cell lysates (50 μg) for mGluR1a. The bar graph shows the densitometric analysis of biotin-labeled cell surface mGluR1a immunoblots normalized to total mGluR1a biotinylation. Data represents the mean ± SD of 7 independent experiments. *P < 0.05 versus GFP transfected cells. B) Upper blot shows a representative immunoblot for cell surface biotin-labeled mGluR5a in HEK 293 cells transfected with 3 μg of pcDNA3.1 encoding FL-mGluR5a along with 0.5 μg of plasmid cDNA encoding either GFP or GFP-CaMKIIα following 50 μM quisqualate treatment. Lower blot shows the total cell lysates (50 μg) for mGluR5a. The bar graph shows the densitometric analysis of biotin-labeled cell surface mGluR1a immunoblots normalized to total mGluR5a biotinylation. Data represents the mean ± S.E.M. of 4 independent experiments completed in duplicate. *P < 0.05 versus GFP transfected cells, #P < 0.05 versus GFP CaMKIIα control.

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