Figure 6

Aβ42 oligomers stimulate ERK1/2 phosphorylation in an mGluR5a and PKC dependent manner that is independent of CaMKIIα overexpression. A) Shown are representative immunoblots of p-ERK1/2 activity and total ERK1/2 expression in HEK 293 cells transfected with 2 μg of pcDNA3.1 encoding FLAG-mGluR5a along with either 0.5 μg of plasmid cDNA encoding either GFP or CaMKIIα and treated with 100 nM Aβ42 oligomer for 5 and 10 min. Bar graph shows the densitometric analysis of ERK1/2 phosphorylation normalized to both basal and total ERK1/2 protein expression. Data represents the mean ± SD of 4 independent experiments. *P < 0.05 versus non-transfected (NT) cells. B) Shown are representative immunoblots of p-ERK1/2 activity and total ERK1/2 expression in HEK 293 cells transfected with 2 μg of pcDNA3.1 encoding FLAG-mGluR5a and treated with either 50 μM quisqualate or 100 nM Aβ42 oligomer in either the presence or absence of 1 μM Bis-1. Bar graph shows the densitometric analysis of ERK1/2 phosphorylation normalized to both basal and total ERK1/2 protein expression. Data represents the mean ± SD of 7 independent experiments. *P < 0.05 versus non-transfected (NT) cells.