Effect of mGluR5a and PrP
expression of GFP-CaMKIIα subcellular localization following agonist stimulation. HEK 293 cells were transiently transfected with different combinations of GFP-CaMKIIα (0.5 μg), FLAG-mGluR5a (2 μg), PrPC (2 μg) or empty pEGFP (2 μg) pcDNA3.1 plasmid cDNA. Shown are representative confocal microscopic images of HEK 293 cells transfected with A) pEGFP (control) along with both FL-mGluR5a and PrPC, B) GFP-CaMKIIα alone (green), C) GFP-CaMKIIα along with FL-mGluR5a (red), D) GFP-CaMKIIα along with PrPC (blue), and E) GFP-CaMKIIα along with both FL-mGluR5a and PrPC. All transfections were treated with 30 μM quisqualate for 20 min. F) Quantification of the number of cells exhibiting GFP-CaMKIIα puncta, number of cells imaged is shown in brackets. Data is representative 4 different experiments.