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Figure 4 | Molecular Brain

Figure 4

From: The protein phosphatase activity of PTEN is essential for regulating neural stem cell differentiation

Figure 4

Expression of wild-type, but not the protein phosphatase mutant form of PTEN, rescues the accelerated differentiation induced by the genetic deletion of PTEN. (A) Pten loxP/loxP NSCs were co-transduced with Cre-GFP and either GFP, or HA-tagged WT PTEN, Y138L, G129E, or C124S constructs; co-expression of the two constructs was visualized by simultaneously labeling cells with antibodies against GFP and HA. The mutations are described in the Figure 2 legend. Scale bar = 40 μm. (B) Overexpression of WT PTEN abolished the increase in the number of Tuj1+ cells induced by the loss of PTEN (Cre-GFP + HA PTEN-WT). G129E, which retains protein phosphatase activity, also rescued the precocious differentiation. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01 (n > 200 cells per group from each experiment).

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