ICH-induced brain injury volume is reduced in TLR2 KO mice. WT and TLR2 KO mice were treated with ICH by collagenase injection (0.075 U in 0.5 μl PBS). (A) At 6 h following PBS or collagenase injection, total RNA was prepared from ipsilateral hemorrhagic tissue in WT mice (n = 3) and used for quantitative real-time RT-PCR to measure TLR2 mRNA level. Data are expressed as mean ± SEM (*** p < 0.001, vs. PBS-injected WT mice). (B-E) At 24 h after collagenase injection, the brains were sectioned and stained with TLR2 antibody. Scale bar: 50 μm. WT and TLR2 KO mice (n = 5) were injected with either PBS (F and
G) or collagenase (H-K), and the brains were sectioned and stained with cresyl violet at 24 or 72 h following injection. Representative pictures are shown. Scale bar: 200 μm. (L) Injury volume (mm3) was calculated by multiplying section thickness and injured hemorrhagic areas. Data are expressed as mean ± SEM (** p < 0.01, * p < 0.05, vs. collagenase-injected WT mice, n = 5).