Figure 4

TLR2 signaling contributes to collagenase-induced BBB damage. (A-H) WT and TLR2 KO mice (n = 6) were injected with Evans blue dye 2 days after ICH. ICH-injured brains were sectioned at 3 days, and evaluated with either cresyl violet staining (A, C, E, and G) or Evans blue staining under light microscope (B, D, F, and H). Serial sections of two different brain regions were stained: AP, −0.85 (C, D, G, and H) and AP, −0.45 (A, B, E, and F). Representative pictures are shown. The Evans blue-stained brain volume was calculated and presented in a graph (I). Data are expressed as mean ± SEM (* p < 0.05, vs. collagenase-injected WT mice).