TLR2 signaling induces MMP9 activity in astrocytes. (A-F) Primary cerebral glia cultures prepared from WT and TLR2 KO mice were immunostained with anti-GFAP (A and
D) and TLR2 (B and
E) antibodies. Merged pictures are shown (C and
F). (G) Primary astrocytes were prepared from WT and TLR2 KO mice and stimulated with Pam3 (2 μg/ml). After 24 h, medium was collected and MMP9 activity was determined by gel zymography. (H) MMP9 transcript was determined by real-time RT-PCR in primary astrocytes at 6 h after Pam3 stimulation. Data are presented as mean ± SEM (* p < 0.01).