Figure 8

Neutrophil-attracting chemokine and adhesion molecule expression are attenuated in TLR2 KO mice. WT and TLR2 KO mice were subjected to collagenase-induced ICH. Six h post-ICH, total RNA was isolated from ipsilateral hemorrhagic tissue (AP, 0.0 to −2.0 mm) and used for quantitative real-time RT-PCR to measure CXCL1 and CXCL2 (A), CCL2 (B), and ICAM-1 (C) mRNA levels. Data are expressed as mean ± SEM (** p < 0.01, * p < 0.05, vs. collagenase-injected WT mice, n = 5). (D-O) Brain sections of WT (D-F, J-L) and TLR2 KO (G-I, M-O) mice (n = 3) were immunostained with anti-GFAP (E, F, H, I, K, L, N, and O) and anti-CXCL1 (D, G, F, and I) or anti-CXCL2 (J, M, L, and O) antibodies 24 h after collagenase injection. Scale bars: 25 μm.