Figure 12

Lack of carbachol-induced phosphorylation of ERK1/2 in hippocampi from Aldh2 ^-/- mice. Hippocampal slices from 6 month old wildtype and Aldh2 ^-/- mice were incubated with 50 μM carbachol (+) or vehicle (-) for 30 min and snap frozen. Immunoblot analysis was performed using 30 μg protein of hippocampal homogenate, and immunoreactive bands were quantitated by densitometry. Data are presented as the mean ± SD (n = 6 mice) and were analyzed by Student’s t-test for unpaired data. ***, significant difference from basal (p < 0.001).