Figure 9
Age-related decreases in phospho-GSK3β (Ser9) in hippocampi from Aldh2 -/- mice. Immunoblot analysis was performed on hippocampal homogenates (30 μg protein) from 3, 6, 9 and 12 month old wildtype or Aldh2 -/- mice and probed for phosphorylated GSK3β and β-actin. A representative immunoblot is shown from 9 month animals. Immunoreactive bands were quantitated by densitometry. Data are presented as the mean ± SD (n = 4 mice) and were analyzed by a two-way ANOVA with a Bonferroni post-hoc test. *, significant differences from wildtype (**p < 0.01, ***p < 0.001).