Table 1 SHIRPA standardized battery for 2-3 month wildtype and Aldh2 ^-/- mice

Viewing jar

Body position

Sitting or Standing: 13 (100%)

Sitting or Standing: 20 (100%)

Spontaneous activity

Casual Scratch, Slow Movement 13 (100%)

Casual Scratch, Slow Movement 20 (100%)

Respiration rate

Normal: 13 (100%)

Normal: 20 (100%)

Body tremor

Normal: 13 (100%)

Normal: 20 (100%)

Palpebral closure

Open: 13 (100%)

Open: 20 (100%)

Piloerection

Normal: 13 (100%)

Normal: 20 (100%)

Gait

Normal: 13 (100%)

Normal: 20 (100%)

Pelvic elevation

Normal: 13 (100%)

Normal: 20 (100%)

Tail elevation

Horizontal: 13 (100%)

Horizontal: 20 (100%)

Touch escape

Mild 4 (31%)

Mild 7 (35%)

Moderate 9 (69%)

Moderate 13 (65%)

Positional passivity

Struggles when held tail: 13 (100%

Struggles when held tail: 20 (100%)

Trunk curl

Present: 13 (100%)

Present: 20 (100%)

Limb grasping

Present: 13 (100%)

Present: 20 (100%)

Grip strength

Active Grip, Effective: 13 (100%)

Active Grip, Effective: 20 (100%)

Body tone

Slight resistance: 13 (100%)

Slight resistance: 20 (100%)

Pinna reflex

Active Retraction: 13 (100%)

Active Retraction: 20 (100%)

Wire maneuver

Difficulty to Grasp with Hindlegs: 2 (15%)

Difficulty to Grasp with Hindlegs: 4 (20%)

Active Grip with Hind Legs: 11(85%)

Active Grip with Hind Legs: 16 (80%)

Skin color

Pink: 13(100%)

Pink: 20(100%)

Abdominal tone

Slight resistance: 13 (100%)

Slight resistance: 20 (100%)

Lacrimation

None 13 (100%)

None 20(100%)

Provoked biting

Present 13 (100%)

Present 20(100%)

Negative geotaxis

Turns and Climbs Grid 13(100%)

Turns and Climbs Grid 20(100%)

Handling behaviour

Irritability

Present 13(100%)

Present 20 (100%)

Aggression

Present 13 (100%)

Present 20 (100%)

Visual and auditory proficiency

Visual placing test

Before Vibrasse Contact (Visual): 13 (100%)

Before Vibrasse Contact (Visual): 20 (100%)

Preyer’s reflex test

Positive Response to Clap: 13 (100%)

Positive Response to Clap: 20 (100%)