RNA-seq analysis of differentially expressed genes in E14.5 Ptf1a
Cre/Cre retinas. (A) Cluster analysis reveals a large group of significantly down-regulated genes and a smaller group of significantly upregulated genes in the mutant retina. (B) Volcano plot (significance vs fold change) of significantly altered genes (fold change ≥ 2 and p < 0.05). (C) Differentially expressed genes grouped by molecular function. Cyan indicates downregulated genes and yellow/orange upregulated genes. GPCR, G-protein coupled receptor; NR, ligand-dependent nuclear receptor; TF, transcription factor; TMR, transmembrane receptor. (D) Representative functional GO terms significantly enriched for the differentially expressed genes. (E) Representative transcription factor genes whose expression is significantly altered in the mutant retina.