Figure 4
From: Tfap2a and 2b act downstream of Ptf1a to promote amacrine cell differentiation during retinogenesis
Effect of Ptf1a misexpressed at E13.5 on the formation of different retinal cell types. (A) Schematics of control-GFP and Ptf1a-GFP retroviral constructs. The internal ribosomal entry site (IRES) allows for efficient expression of both Ptf1a and GFP. (B) Quantitation of GFP+ cells that become immunoreactive for various cell type-specific markers. Each histogram represents the mean ± SD for three retinas. More than 500 GFP+ cells were scored in each retina. (C-N) Sections from retinas infected with control-GFP or Ptf1a-GFP viruses were double-immunostained with an anti-GFP antibody and antibodies against the indicated cell type-specific markers. Forced Ptf1a expression in E13.5 retinas led to an increase in amacrine cells immunoreactive for Pax6, GLYT1 or GAD67 (C-H) and horizontal cells immunoreactive for Lim1 (I, J), but a decrease of ganglion cells immunoreactive for Brn3a or Brn3b (K-N). Arrows point to representative colocalized cells and insets show corresponding outlined regions at a higher magnification. Abbreviations: GCL, ganglion cell layer; ONL, outer nuclear layer. Scale bar: I-N, 39.7 μm; C-H, 34 μm.