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Fig. 4 | Molecular Brain

Fig. 4

From: Differentiation of multipotent neural stem cells derived from Rett syndrome patients is biased toward the astrocytic lineage

Fig. 4

Comparison of acquired neuronal and astrocytic properties between MeCP2-positive and negative hiPSC-derived neural cells. (A) Immunostaining images of neural cells. Double labeling for GFAP (red) and βIII-tubulin (green) is shown along with Hoechst staining (Ho, blue) in RS1-52 M, RS1-61P, RS2-65 M, and RS2-62P RTT-hiPSC-derived neurons. Scale bar, 200 μm. (B) Fraction of GFAP-positive cells relative to Hoechst-positive cells in neural cells differentiated from RTT-hiPSC lines. (C) A qPCR-facilitated comparison of gene expression for astrocytic markers (GFAP, S100β) and neuronal markers (TUBB3, MAP2) in neural cells differentiated from RTT-hiPSC lines. Relative gene expression levels were normalized to that of ACTB. Data in (B) and (C) were analyzed by Student’s t-test and Welch’s t-test (*p < 0.05)

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