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Fig. 5 | Molecular Brain

Fig. 5

From: Differentiation of multipotent neural stem cells derived from Rett syndrome patients is biased toward the astrocytic lineage

Fig. 5

MeCP2 binding analysis and DNA methylation status of the GFAP promoter region. (A) Schematic representation of the hGFAP genomic locus. ChIP/qPCR analysis was performed for the genomic regions (i.e., the STAT3 binding site and GFAP exon 1) indicated by the blue bidirectional arrows. Bisulfite sequencing was performed for the genomic region indicated by the black bidirectional arrows. (B) MeCP2 binding to the STAT3 binding site within the GFAP promoter region was quantified via ChIP/qPCR analysis by using an anti-MeCP2 antibody in neural cells differentiated from RTT-hiPSC lines. (C) Methylation frequencies of (1) the CpG site within the STAT3 recognition sequence and (2) seven other CpG sites around this sequence were analyzed in hiPSC-derived neurosphres via bisulfite sequencing

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Molecular Brain

ISSN: 1756-6606