Fig. 3
Depolarizing shift of EGABA in the MNCs of multiparous lactating rats. (a) Estimation of EGABA with the use of the currents elicited by focally applied muscimol (10 μM, 10 ms) at various holding potentials (VH) in the MNCs of virgin (inset; upper left) and Lac3 rats (inset; lower right). These current traces were obtained after the blockade of fast sodium current and glutamatergic transmission with the cocktail of tetrodotoxin (0.5 μM), AP-5 (100 μM) and DNQX (20 μM). Peak amplitudes of the muscimol-elicited currents are plotted against VH. Linear regression was used to fit the data points. The intersections (
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) of the regression lines with the abscissa were taken as the reversal potentials of the muscimol-elicited responses (i.e., EGABA’s). (b) Dot plots and bar graphs showing the ranges and means (±SEM) of the EGABA’s of the MNCs of Virgin, Lac1, Lac3 and Dry3 rat groups. Holm-Sidak all pairwise comparison tests performed after one-way ANOVA (p < 0.001) indicated that these rat groups were different from one another in the mean EGABA. This was denoted with different symbols associated with the bar graphs. n: the number of cells examined