Fig. 5

Reduced progenitor cells in PAK1 KO mice. Coronal brain sections of WT and PAK1 KO E14.5 embryos were stained for the active form of caspase 3 (CC3) or Pax6. a, b CC3 (red) and DAPI (blue) staining of WT (a) and PAK1 KO mice (b). c Summary graph of CC3 positive cells showing no differences between WT and PAK1 KO mice. d-g Pax6 (red) and DAPI (blue) staining of WT (d, f) and PAK1 KO mice (e, g). h Summary graph showing significantly decreased numbers of Pax6 positive cells PAK1 KO mice compared to their WT littermates. ***p < 0.001. Scale bars: 100 μm