Fig. 7

Altered cell cycle state in the telencephalon of PAK1 KO embryos. a-f Pregnant mice were injected with BrdU at E13.5 for 24 h and the embryos of the WT (a-c) and PAK1 KO (d-f) were then dissected at E14.5 and processed for immunostaining for BrdU (green), Ki67 (red) and DAPI (blue). g, h Summary graphs of the percentages of BrdU+/Ki67+ (g) and BrdU+/Ki67- (h) cells relative to the total BrdU+ cells showing a significantly decreased number of cells staying in the active phases of the cell cycle, as indicated by reduced BrdU+/Ki67+ cells and increased BrdU+/Ki67- cells, in PAK1 KO mice compared to the WT littermates. (i) Summary graph showing that the number of total Ki67 positive cells was also decreased in the dorsal telencephalon in the PAK1 KO mice compared to the WT littermates. **p < 0.01, ***p < 0.001. Scale bars: 100 μm. CP, cortical plate; VZ, ventricular zone; SVZ, subventricular zone; IZ, intermediate zone