Figure 12

Model depicting the composition and organization of Nr2e1 ^+/+ and Nr2e1 ^frc/frc retinal cells at P7. a Wild-type retina with 3 nuclear layers and 2 plexiform layers. b Nr2e1 ^frc/frc retinas contain reduced numbers of ganglion, bipolar, and rod cells, but increased numbers of glycinergic amacrine cells. Disorganization of the IPL is evident, as well as the presence of an ectopic plexiform layer (EPL) in the INL. Müller glia misexpress GFAP and Brn3a, and have somas localized closer to the OPL intermingled with bipolar cells. c A predominantly Nr2e1 ^frc/frc clone of cells in a Wt↔frc chimera has reduced numbers of bipolar and rods, but increased number of amacrine cells. Ganglion cell numbers are restored to wild type. There is also an increase in S-cone numbers compared to a wild-type clone. Disorganization of the IPL, and an EPL, are still evident. Müller glia misexpress Brn3a but not GFAP, and have mispositioned somas. Ganglion cells are ectopically positioned in the IPL and INL. EPL, ectopic plexiform layer; GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; Neg., negative; ONL, outer nuclear layer; OPL, outer plexiform layer; Pos., positive