Figure 2
Nr2e1frc/frc-Müller-glia misexpression of GFAP and retinal structural defects were rescued in Wt↔frc chimeras. Transverse retinal sections from P21 mice were immunostained for GFAP (red). a GFAP was observed in the GCL of wild-type and Nr2e1 frc/frc retinas but also in the processes of Müller glia in Nr2e1 frc/frc retinas (arrow). b Nr2e1 frc/frc retinas had abnormal INL intrusions into the ONL (asterisk). c A Wt↔frc chimera with 86 % Nr2e1 frc/frc cells (EGFP positive, green) showed absence of GFAP expression in Müller cells and absence of INL intrusions into the ONL. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; Hoechst, nuclear counterstain (blue); scale bar = 50 μm. n = 9 for Wt↔Wt, n = 10 for Wt↔frc