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Figure 3 | Molecular Brain

Figure 3

From: Nr2e1 regulates retinal lamination and the development of Müller glia, S-cones, and glycineric amacrine cells during retinogenesis

Figure 3

Nr2e1 frc/frc P7 retinas had reduced numbers of ganglion cells that were rescued in Wt↔frc chimeras. Transverse retinal sections from P7 Nr2e1 +/+, Nr2e1 frc/frc, and chimeric mice were immunostained for Brn3a (ganglion cells). a Nr2e1 frc/frc retinas had less Brn3a positive cells (green)in the GCL and misexpressed Brn3a in cells ofthe ventral INL that resembled Müller glia b Magnification of the rectangle in A showing Brn3anuclear staining in some GCL cell nuclei (arrow heads) and cytoplasmic projectionsresembling Müller glia end-feet c Ganglion cells were counted throughout five sections across the retina of Nr2e1 +/+ and Nr2e1 frc/frc mice. Numbers were normalized to retinal length and expressed as percentages of Nr2e1 +/+ cell numbers. Reduced numbers of ganglion cells were observed in Nr2e1-mutant retinas compared to wild type (63 % decrease). d In Wt↔Wt and Wt↔frc chimeras, the density of EGFP positive (green) ganglion cells (arrows), appeared similar to the density of EGFP negative ganglion cells. Representative images of a 71 % Wt↔Wt and a 58 % Wt↔frc chimeric retina are shown. Brn3a positive cells were mislocalized in the IPL (arrowheads) and INL of Wt↔frc retinas. e Quantification of the density of ganglion cells that were derived from host blastocyst or ESCs in chimeras was assessed by counting single-labeled cells (Brn3a positive and EGFP negative) or double-labeled cells (Brn3a positive and EGFP positive) and dividing them by the EGFP negative or EGFP positive retinal area (ONL + INL), respectively. The density of Nr2e1 frc/frc ganglion cells (EGFP positive) was similar to the density of wild-type ganglion cells (EGFP negative) in Wt↔frc chimeras. n = 3 for Nr2e1 +/+, n = 3 for Nr2e1 frc/frc, n = 3 for Wt↔Wt, n = 3 for Wt↔frc;*, P ≤ 0.05; ns, not significant; error bars represent SEM. GCL, ganglion cell layer; Hoechst, nuclear counterstain (blue); INL, inner nuclear layer; neg., negative; ONL, outer nuclear layer; pos., positive; scale bar = 50 μm

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