Figure 6

Nr2e1 ^frc/frc retinas had normal numbers of horizontal, cone, and Müller glia cells. Transverse retinal sections from P7 and P21 Nr2e1 ^+/+ and Nr2e1 ^frc/frc mice were immunostained for calbindin (horizontal cells, adjacent to the ONL), arrestin (cones), and SOX-9 (Müller glia). a-c P7 retinal sections showing (a) horizontal cells in green, (b) cones in red, and (c) Müller glia in green. d,e Each retinal cell type was counted throughout five sections across the retina of (d) P7 or (e) P21 Nr2e1 ^+/+ and Nr2e1 ^frc/frc mice. Numbers were normalized to retinal length and expressed as percentages of Nr2e1 ^+/+ cell numbers. Normal numbers of horizontal, cone, and Müller glia cells were observed in Nr2e1-mutant retinas compared to wild type at both time-points. n = 3 for Nr2e1 ^+/+, n = 3 for Nr2e1 ^frc/frc; *, P ≤ 0.05; ns, not significant; error bars represent SEM. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; scale bar = 50 μm