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Figure 8 | Molecular Brain

Figure 8

From: Nr2e1 regulates retinal lamination and the development of Müller glia, S-cones, and glycineric amacrine cells during retinogenesis

Figure 8

Nr2e1 frc/frc retinas displayed an ectopic plexiform layer that was not rescued in Wt↔frc chimeras. Transverse retinal sections from P7 Nr2e1 +/+, Nr2e1 frc/frc, and chimeric mice were immunostained for PKC-alpha or syntaxin-1A. a A region devoid of Hoechst-stained nuclei indicated an ectopic plexiform layer (EPL) in Nr2e1 frc/frc retinas (open arrow). b Magnification of the box in A showing the EPL (open arrow). c PKC-alpha positive bipolar cells (green) extended processes into the IPL in wild-type retinas but also to the EPL (open arrow) in Nr2e1-mutant retinas. d Magnification of the box in G showing bipolar cell processes (solid arrow) in the EPL (open arrow). e Chimeras showing the distribution of syntaxin-1A (red) in the INL. Representative images of a 51 % Wt↔Wt and a 58 % Wt↔frc chimeric retina are shown. Note that syntaxin-1A positive EPL (open arrow) was seen in Wt↔frc chimeras in regions enriched with Nr2e1-mutant cells (EGFP positive, green). The solid arrows show a region with predominantly wild-type cells (EGFP negative) where the EPL is absent. Also note that the IPL (brackets) from Wt↔frc retina is thicker and more disorganized compared to Wt↔Wt. n = 3 for Nr2e1 +/+, n = 3 for Nr2e1 frc/frc, n = 4 for Wt↔Wt, n = 4 for Wt↔frc; EPL, ectopic plexiform layer; GCL, ganglion cell layer; Hoechst, nuclear counterstain (blue); INL, inner nuclear layer; ONL, outer nuclear layer; OPL, outer plexiform layer; scale bar = 50 μm

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