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Figure 9 | Molecular Brain

Figure 9

From: Nr2e1 regulates retinal lamination and the development of Müller glia, S-cones, and glycineric amacrine cells during retinogenesis

Figure 9

P21 retinas show lamination defects and loss of amacrine cells. Transverse retinal sections from P21 Nr2e1 +/+, Nr2e1 frc/frc, and chimeric mice were immunostained for syntaxin-1A or GlyT1. a In wild-type retinas, syntaxin-1A positive cells (green) were located in the INL with processes that projected into the IPL. In P21 Nr2e1-mutant retinas, syntaxin-1A positive processes projected to both the IPL and the EPL (open arrow). Note a decrease in syntaxin-1A positive cell bodies. b Magnification of the box in A showing the EPL (open arrow) of Nr2e1-mutant retinas positive for syntaxin-1A (solid arrow). c The glycinergic marker GlyT1 (red) revealed a reduction in the number of this subtype of amacrine cells in Nr2e1 frc/frc retinas. d Magnification of the box in C showing the presence of many interplexiform amacrine cells extending neurites to the OPL in Nr2e1 frc/frc retinas (solid arrow) indicative of additional lamination defects. In P21 chimeras, (e) the presence of many GlyT1 positive cells (red) in both Wt↔Wt and Wt↔frc is evident. Representative images of a 46 % Wt↔Wt and a 62 % Wt↔frc chimeric retina are shown. f,g Magnification of the boxes in E showing (f) not detectable interplexiform cells in the 46 % Wt↔Wt chimera and (g) the presence of strongly labeled interplexiform cells in the 62 % Wt↔frc chimera (asterisks). h Magnification of dotted boxes in E showing Nr2e1-mutant cells (EGFP positive, green) that are also GlyT1 positive (red) in the INL (exemplified by arrows). n = 3 for Nr2e1 +/+, n = 3 for Nr2e1 frc/frc, n = 3 for P21 Wt↔Wt, n = 3 for P21 Wt↔frc; EPL, ectopic plexiform layer; GCL, ganglion cell layer; Hoechst, nuclear counterstain (blue); INL, inner nuclear layer; ONL, outer nuclear layer; OPL, outer plexiform layer; scale bar in A to E = 50 μm; scale bar in F = 7 μm; scale bar in H = 9 μm

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