Fig. 1

PIWIL1 regulates cortical radial migration. a-f Effect of knockdown of PIWIL1 in rat cortical neurons by IUE with plasmids coding for PIWIL1 siRNA (RNAi 1). Coronal brain sections at different stages were stained with anti-GFP (green) and DAPI (red). g-l Distribution of labeled cells in brains electroporated with RNAi 1 (g-i) and RNAi 4 (j-l) or control constructs (Scramble). m Different forms of truncated human PIWIL1 (HIWI) or mutated mouse PIWIL1. n-s, u Only constructs containing both PAZ and PIWI domains rescued the migration. The Scramble control data were from the experiment in Additional file 2: Figure S2E. CAG, pCAG-IRES-GFP vector as control. t, u Co-electroporation of D633A-Res with RNAi 2 rescued the migration defect. Scale bar, 250 μm. Error bar, SEM. *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test)