Fig. 4
From: Regulation of density of functional presynaptic terminals by local energy supply
Elevating [Mg2+]i leads to increased Ca2+-sensitivity-related proteins in terminals. (a) Schematic cartoon illustrates the three critical biophysical factors affecting presynaptic vesicle release. (b and c) Immunofluorescence. No change of quantity of Synapsin1, Cav2.1 and Cav2.1 (b), but remarkable increase in Ca2+-sensitivity-related proteins at dendritic branches (c) after elevating [Mg2+]o for 4 hr. Pseudo-color scale: fluorescent intensity. (d) Elevating [Mg2+]o for 4 hr led to increase in Ca2+-sensitivity-related protein positive fluorescence in terminals at dendritic branches (n = 10–21 coverslips for each point). The mean ± SEM of coverslips was presented. Two-tailed Student’s t-test, comparing 1.2 4hr to Ctrl 0.8, n.s. no significance, *** p < 0.001