Skip to main content
Fig. 6 | Molecular Brain

Fig. 6

From: Regulation of density of functional presynaptic terminals by local energy supply

Fig. 6

Elevating [Mg2+]i increases general mitochondrial function and intracellular ATP. (a) The mitochondrial potential (ΔΨ) was determined by the ratio of aggregate and monomer of JC-1 fluorescence. (b) Both mitochondrial density (N mito ) and general mitochondrial function (N mito  × ΔΨ) in distal branches were significantly enhanced after elevating Mg2+ level for 4 hr (19.4 ± 2.9 % and 67.8 ± 12.4 %, n = 14 coverslips) and for long term (>48 hr) (19.6 ± 2.1 % and 56.2 ± 6.5 %, n = 20 coverslips). Dotted line represents initial value of N mito , N mito  × ΔΨ and ΔΨ normalized to 100 %. (c) Intracellular ATP concentration ([ATP]i) was linearly correlated with N mito  × ΔΨ at equilibrium (altering [Mg2+]o for > 12 hr) (n = 7 coverslips). (d) Absolute temporal changes of N mito (ΔN mito ) and [Mg2+]i (Δ[Mg2+]i) exhibited a linear correlation, either by elevating [Mg2+]o or by administering 1 μM Imipramine (IM) for 2–4 hr (n = 5–11 coverslips). Each point represented the changes of both ΔN mito and Δ[Mg2+]i (using sister cultures from the same batch) at different time after a treatment, and the colored numbers beside each point indicated the hours after the treatment. These data were collected from 2 individual batches of measurements. The mean ± SEM of coverslips was presented. Two-tailed Student t-test for (b) comparing both 1.2 4hr and 1.2 LT to Ctrl 0.8, *** p < 0.001. Linear regression for (c and d)

Back to article page