Fig. 8
From: Regulation of density of functional presynaptic terminals by local energy supply
Quantity of Ca2+-sensitivity-related proteins versus density of mitochondria in intact animals. (a) Electron Microscopy (EM) images of Hippocampus CA1 Stratum Radiatum region (HP/CA1/SR) from 24-months-old Control and MgT-supplemented (for 8 months) rats (m, mitochondrion [red colored]; s, synapse). (b) Density of mitochondria (N mito , number of mitochondria per area of 70 nm brain slice) in MgT group (0.47 ± 0.02 μm−2, n = 11 rats) was 25.9 % higher than Control group (0.37 ± 0.02 μm−2, n = 9 rats) (Kolmogorov-Smirnov test, p < 0.01). (c) Immunostained structure-related protein SYP and Ca2+-sensitivity-related proteins in 70 nm ultrathin slices from adjacent tissue blocks in HP/CA1/SR of the same rats as in (a and b). Quantity of Ca2+-sensitivity-related proteins was higher in MgT group than in Control group. Pseudo-color scale: fluorescent intensity. (d) Normalized total quantity of each protein (Q) was calculated for each rat. Q SYP was not correlated with N mito (d lower) Total amount of Ca2+-sensitivity-related proteins (ΣQ proteins ) was linearly correlated with N mito for both groups and the correlation curve was positively shifted in MgT group (d upper) (Kolmogorov-Smirnov test, p < 0.0005). (e) Relative quantity of Ca2+-sensitivity-related proteins to SYP, i.e. ΣQ proteins /Q SYP , was linearly correlated with N mito , and the linear correlation was positively shifted in MgT group (Kolmogorov-Smirnov test, p < 0.003). Linear regression for (d and e)