Fig. 1

Tissue sampling for 2-D DIGE experiments. a Illustration of the tissue sampling in primary area 17: a frontal section of an in situ hybridization for zif268 in area 17 of a 2-month old normal kitten and the relevant level of the Rosenquist retinotopic map (1985). We have collected brain tissue from the central (C) and peripheral (P) visual field representation (White/black boxes) at Horsley-Clarke coordinate posterior 7 (P7.0). White/black lines demarcate the areal borders of area 17. Scale bar: 1 mm. b Visualization of all differential spots on an image of a preparative 2-D gel. Spot numbers match with the information in Table 1. Mw: molecular weight. c Venn diagrams illustrating the number of age-related differential spots as a function of cortical region (central or peripheral area 17) for normal and BD cats