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Table 2 Immunohistochemistry antibody source and specificity

From: Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors

Antibody

Species, dilution, incubation

Antigen retrieval

IRS1

Rabbit IgG, 1/25, 1Ā h RT

TSC, pHĀ 6.5, MW 10Ā min

pIRS1 S616

Rabbit IgG, 1/100, 1Ā h RT

TSC, pHĀ 6.5, MW 10Ā min

pIRS1 S636/639

Rabbit IgG, 1/50, 1Ā h RT

TSC, pHĀ 6.5, MW 10Ā min

pIRS1 Y612

Rabbit IgG, 1/100, 1Ā h RT

EDTA, pHĀ 8.0, MW 10Ā min

GFAP

Rabbit IgG, 1/500, ON, 4Ā Ā°C

Dependent on initial probe (above)

  1. TSC tris-sodium citrate, EDTA ethylenediaminetetraacetic acid, RT room temperature, ON overnight, MW microwave