Antibody | Species, dilution, incubation | Antigen retrieval |
---|---|---|
IRS1 | Rabbit IgG, 1/25, 1Ā h RT | TSC, pHĀ 6.5, MW 10Ā min |
pIRS1 S616 | Rabbit IgG, 1/100, 1Ā h RT | TSC, pHĀ 6.5, MW 10Ā min |
pIRS1 S636/639 | Rabbit IgG, 1/50, 1Ā h RT | TSC, pHĀ 6.5, MW 10Ā min |
pIRS1 Y612 | Rabbit IgG, 1/100, 1Ā h RT | EDTA, pHĀ 8.0, MW 10Ā min |
GFAP | Rabbit IgG, 1/500, ON, 4Ā Ā°C | Dependent on initial probe (above) |