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Table 2 Immunohistochemistry antibody source and specificity

From: Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors

Antibody Species, dilution, incubation Antigen retrieval
IRS1 Rabbit IgG, 1/25, 1 h RT TSC, pH 6.5, MW 10 min
pIRS1 S616 Rabbit IgG, 1/100, 1 h RT TSC, pH 6.5, MW 10 min
pIRS1 S636/639 Rabbit IgG, 1/50, 1 h RT TSC, pH 6.5, MW 10 min
pIRS1 Y612 Rabbit IgG, 1/100, 1 h RT EDTA, pH 8.0, MW 10 min
GFAP Rabbit IgG, 1/500, ON, 4 °C Dependent on initial probe (above)
  1. TSC tris-sodium citrate, EDTA ethylenediaminetetraacetic acid, RT room temperature, ON overnight, MW microwave