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Fig. 2 | Molecular Brain

Fig. 2

From: A septo-temporal molecular gradient of sfrp3 in the dentate gyrus differentially regulates quiescent adult hippocampal neural stem cell activation

Fig. 2

A gradient of sfrp3 expression in the dentate gyrus of adult mouse hippocampus. a Mid-sagittal section of dissected mouse hippocampus showing in situ hybridization (ISH) for sfrp3 mRNA. Scale bar, 500 μm. b Quantitative analysis of sfrp3 expression by quantitative real-time PCR. Values present mean ± S.E.M. (n = 4; * < 0.05; ANOVA). c. Representative images of ISH for sfrp3 mRNA on twelve serial sections along the septo-temporal axis, with the planes oriented as shown in Fig. 1a. Scale bar, 10 μm. d Quantification of signal intensity for sfrp3 in situ, which was performed on the images shown in Fig. 2c. Open circles, diamonds and triangles represent sfrp3 mRNA from septo-temporal axis of three individual mice. Closed circles are the mean of the three mice. Values represent mean ± S.E.M. (n = 3). e Representative images of sfrp3 mRNA in situ in the dentate gyrus of adult sfrp3 knockout (−/−), heterozygous (+/−), and wild-type (+/+) littermates. Scale bar, 500 μm

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Molecular Brain

ISSN: 1756-6606