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Fig. 4 | Molecular Brain

Fig. 4

From: A septo-temporal molecular gradient of sfrp3 in the dentate gyrus differentially regulates quiescent adult hippocampal neural stem cell activation

Fig. 4

Activation of quiescent radial glia-like neural stem cells increases in adult sfrp3 knockout (KO) mice. a Quantitative analysis of MCM2+nestin+ RGLs along the septo-temporal axis of adult sfrp3 KO and wildtype (WT) littermates. Values present mean ± S.E.M. [n = 6 (KO), 7 (WT)]. b Higher relative increase in RGL activation in the temporal dentate gyrus of adult sfrp3 KO mice. Same groups of animals in Fig. 4b were analyzed. Data were normalized to control WT group from septal (1) to temporal (4) dentate gyrus. c Representative confocal images of immunostaining of GFP (green) for quiescent and activated clones in the adult nestin-CreER T2+/− ;Z/EG f/+ ; sfrp3 −/− KO mice and nestin-CreER T2+/− ;Z/EG f/+ ; sfrp3 +/+ control mice. Scale bar = 10 μm. d Quantification of increased RGL activation at both septal and temporal poles in the dentate gyrus of adult sfrp3 KO mice examined at 7 dpi. Numbers associated with the bar graph indicate the number of dentate gyri and total number of clones (in parentheses) analyzed, grey bars = WT, blue bars = KO. Values represent mean ± S.E.M. (*P < 0.01; n.s. P > 0.1; AVOVA). e Stronger relative increase of RGL activation in the temporal dentate gryus of adult sfrp3 KO mice. Same groups of animals in Fig. 4d were analyzed. Data were normalized to control WT group

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